ARUP's Laboratory Test Directory

Borrelia burgdorferi C6 Peptide Antibodies, Total by ELISA with Reflex to IgG by Western Blot : 0051045

Mnemonic: C6 CHRON R

Ordering Recommendation: Preferred reflex test to detect Lyme disease in individuals with >4 weeks of clinical symptoms or exposure to tick. Positive/equivocal screen confirmed by Western blot.
Methodology: Semi-Quantitative Enzyme-Linked Immunosorbent Assay/Qualitative Western Blot
Performed: Tue
Reported: 1-9 days
Specimen Required: Collect: Serum separator tube.

Specimen Preparation: Separate serum from cells ASAP or within 2 hours of collection. Transfer 2 mL serum to an ARUP Standard Transport Tube. (Min: 0.2 mL)

Storage/Transport Temperature: Refrigerated.

Unacceptable Conditions: CSF. Contaminated, heat-inactivated, hemolyzed or lipemic specimens.

Stability (collection to initiation of testing): After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year
Reference Interval:
 
Test Number Components Reference Interval
0051044Borrelia burgdorferi C6 Peptide Antibodies, Total by ELISA 0.90 LI or less:  Negative - C6 peptide antibody to B. burgdorferi not detected.
0.91 - 1.09 LI:  Equivocal - Repeat testing in 10-14 days may be helpful.
1.10 LI or greater: Positive - C6 peptide antibody to B. burgdorferi detected.
0050255Borrelia burgdorferi Antibody, IgG by Western Blot Effective August 15, 2011

Negative

Interpretive Data: Refer to report
Note: If C6 Peptide by ELISA is 0.91 LI or greater, then IgG Western blot will be added. Additional charges apply.
CPT Code(s): 86618; if positive, add 86617
Cross References: Lyme Disease (Borrelia burgdorferi C6 Peptide Antibodies, Total by ELISA with Reflex to IgG by Western Blot)