ARUP's Laboratory Test Directory

Lymphocyte Antigen and Mitogen Proliferation Panel with Cytokine Response to Mitogens, 12 Cytokines : 0051584
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Time Sensitive
  


Mnemonic: LAM CYT 12

Methodology: Cell Culture/Multiplex Bead Assay
Performed: Tue-Fri
Reported: 9-10 days
Specimen Required: Patient Prep: Collect control specimen from a healthy individual unrelated to patient at approximately the same time as and under similar conditions to the patient.

Collect: Green (sodium heparin) (patient) AND green (sodium heparin) (control). Also acceptable: Yellow (ACD solution A) (patient) AND yellow (ACD solution A) (control). Patient and control specimens must be
collected within 48 hours of test

Specimen Preparation: Transport 20 mL whole blood (patient) AND 20 mL whole blood (control) in original collection tubes. (Min: 14 mL (patient) AND 14 mL (control)) Do not refrigerate or freeze. LIVE CELLS REQUIRED.
Infant Minimum: 3 mL (patient) AND 14 mL (control).

Storage/Transport Temperature: CRITICAL ROOM TEMPERATURE.

Remarks:

Unacceptable Conditions: Yellow (ACD Solution B). Refrigerated or frozen specimens. Specimens in transport longer than 48 hours.

Stability (collection to initiation of testing): Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
Reference Interval:
Test Number Components Reference Interval
0096056Lymphocyte Antigen and Mitogen Proliferation Panel By report
0051574Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Interferon gamma By report
0051580Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Interleukin 1 beta By report
0051571Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Interleukin 2 By report
0051572Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Interleukin 2 Receptor (Soluble) By report
0051576Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Interleukin 4 By report
0051577Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Interleukin 5 By report
0051581Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Interleukin 6 By report
0051582Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Interleukin 8 By report
0051578Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Interleukin 10 By report
0051579Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Interleukin 13 By report
0051583Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Tumor Necrosis Factor alpha By report

Interpretive Data: Phytohemagglutinin, concanavalin A, pokeweed mitogen, Candida antigen, and tetanus antigen are tested independently in mononuclear cell culture. Cell proliferation in response to mitogens and antigens is determined by 3H-thymidine incorporation. Cytokine production in response to mitogens is determined by multi-analyte fluorescence detection.

Proliferation results are reported as the mitogen and/or antigen stimulated counts per minute (CPM) and a stimulation index (SI) which represents the ratio of the stimulated lymphocyte CPM to an unstimulated control CPM. Cytokine results are reported in pg/mL.

Results are to be used for research purposes or in attempts to understand the pathophysiology of immune, infectious, or inflammatory disorders

See Compliance Statement B: www.aruplab.com/CS
Note: Interpretation comparing the patient results to the simultaneously collected client normal control and the laboratory normal control will be provided by an ARUP medical director.
CPT Code(s): 86353 x5; 83520 x12
Cross References: IFN Gamma (Lymphocyte Antigen/Mitogen Prolif. Panel w/ Cytokine Response to Mitogens, 12 Cytokines), IL-1 Beta (Lymphocyte Antigen/Mitogen Prolif. Panel w/ Cytokine Response to Mitogens, 12 Cytokines), IL-10 (Lymphocyte Antigen/Mitogen Prolif. Panel w/ Cytokine Response to Mitogens, 12 Cytokines), IL-12 (Lymphocyte Antigen/Mitogen Prolif. Panel w/ Cytokine Response to Mitogens, 12 Cytokines), IL-13 (Lymphocyte Antigen/Mitogen Prolif. Panel w/ Cytokine Response to Mitogens, 12 Cytokines), IL-2 (Lymphocyte Antigen/Mitogen Prolif. Panel w/ Cytokine Response to Mitogens, 12 Cytokines), IL-2R (Lymphocyte Antigen/Mitogen Prolif. Panel w/ Cytokine Response to Mitogens, 12 Cytokines), IL-4 (Lymphocyte Antigen/Mitogen Prolif. Panel w/ Cytokine Response to Mitogens, 12 Cytokines), IL-5 (Lymphocyte Antigen/Mitogen Prolif. Panel w/ Cytokine Response to Mitogens, 12 Cytokines), IL-6 (Lymphocyte Antigen/Mitogen Prolif. Panel w/ Cytokine Response to Mitogens, 12 Cytokines), IL-8 (Lymphocyte Antigen/Mitogen Prolif. Panel w/ Cytokine Response to Mitogens, 12 Cytokines), TNF Alpha (Lymphocyte Antigen/Mitogen Prolif. Panel w/ Cytokine Response to Mitogens, 12 Cytokines)