ARUP's Laboratory Test Directory

BRAF Codon 600 Mutation Detection with Reflex to MLH1 Promoter Methylation : 0051750
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Additional Technical Information
  


Mnemonic: BRAF RFLX

Ordering Recommendation: Differentiate Lynch syndrome from sporadic microsatellite unstable (MSI) colorectal cancer. Order prior to germline mutation analysis for tumors demonstrating loss of MLH1 protein expression.
Methodology: Polymerase Chain Reaction/Pyrosequencing
Performed: Sun-Sat
Reported: 10-14 days
Specimen Required: Collect: Tumor tissue.

Specimen Preparation: Formalin fix (10 percent neutral buffered formalin) and paraffin embed tissue. Tissue block will be returned after testing.
Transport tissue block or 5 unstained 5-micron slides in a tissue transport kit (ARUP supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787. (Min: 3 slides). Protect paraffin block and/or slides from excessive heat.

Storage/Transport Temperature: Room temperature or refrigerated. Ship in cooled container during summer months.

Remarks: Include surgical pathology report.

Unacceptable Conditions: Less than 25 percent tumor. Specimens fixed/processed in alternative fixatives (alcohol, Prefer®) or heavy metal fixatives. Decalcified specimens.

Stability (collection to initiation of testing): Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable
Reference Interval:
Test Number Components Reference Interval
BRAF V600E by PCR By report
MLH1 Promoter Methylation by Real-time PCR By report

Interpretive Data: Refer to report.

This test is performed pursuant to an agreement with Roche Molecular Systems, Inc.

See Compliance Statement B: www.aruplab.com/CS
Note: If BRAF codon 600 Mutation Detection is negative, then MLH1 Promoter Methylation will be added. Additional charges apply.
CPT Code(s): 88381, 81210, G0452 If reflexed add 81479
Cross References: MLH1 (BRAF codon 600 Mutation Detection with Reflex to MLH1 Promoter Methylation)