ARUP's Laboratory Test Directory

F-Actin (Smooth Muscle) Antibody, IgG : 0055248

Mnemonic: ACTIN IGG

Methodology: Semi-Quantitative Enzyme-Linked Immunosorbent Assay
Performed: Sun-Sat
Reported: Within 24 hours
Specimen Required: Collect: Serum separator tube

Specimen Preparation: Separate serum from cells ASAP or within 2 hours of collection. Transfer 0.5 mL serum to an ARUP Standard Transport Tube. (Min: 0.3 mL)

Storage/Transport Temperature: Refrigerated.

Unacceptable Conditions: Urine or plasma. Contaminated, heat-inactivated, hemolyzed, icteric, or severely lipemic specimens.

Stability (collection to initiation of testing): After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)
Reference Interval:
Effective May 16, 2011
 
19 Units or less Negative
20-30 Units Weak Positive - Suggest repeat testing in two to three weeks with fresh specimen.
31 Units or greater Positive - Suggestive of autoimmune hepatitis type 1 or chronic active hepatitis.

Interpretive Data: F-actin antibodies have been shown to have greater sensitivity and specificity for autoimmune liver disease than anti-smooth muscle antibodies.
CPT Code(s): 83516
Cross References: Actin Antibody (F-Actin (Smooth Muscle) Antibody, IgG), Anti-Actin Antibody (F-Actin (Smooth Muscle) Antibody, IgG), Anti-F-Actin Antibody (F-Actin (Smooth Muscle) Antibody, IgG), Antiactin Antibody (F-Actin (Smooth Muscle) Antibody, IgG)