IGH-BCL2 (BCL-2/JH) Translocation, t(14;18) by PCR
:0055616
Mnemonic: BCL2C-F
Methodology:
Polymerase Chain Reaction
Performed:
DNA isolation: Sun-Sat; Assay: Sun-Sat
Reported:
2-7 days
Specimen Required:
Collect: Lavender (EDTA) OR bone marrow (EDTA) OR tissue.
Specimen Preparation:Whole blood: Transport 5 mL. (Min: 1 mL) OR Bone marrow: Transport 3 mL. (Min: 1 mL) OR Fresh tissue: Freeze immediately. Transport 100 mg or 0.5-2.0 cm3 tissue. OR FFPE tumor tissue: Formalin fix (10 percent neutral buffered formalin) and paraffin embed tissue. Protect from excessive heat. Transport tissue block or four 10-micron shavings. Tissue block will be returned after testing. Transport block(s) and/or shavings in a tissue transport kit (ARUP supply #47808) available online through eSupply using ARUP Connect™or contact ARUP Client Services at (800) 522-2787.
Storage/Transport Temperature:Whole blood OR Bone marrow: Refrigerated. Fresh tissue: Frozen on dry ice. FFPE tumor tissue: Room temperature or refrigerated. Ship in cooled container during summer months.
Unacceptable Conditions:Whole blood OR Bone marrow: Frozen specimens. Clotted or grossly hemolyzed specimens. FFPE tumor tissue: Specimens fixed/processed in alternative fixatives or heavy metal fixatives (B-4 or B-5) or tissue sections on slides. Decalcified specimens.
Stability (collection to initiation of testing):Whole blood OR Bone marrow: Ambient: 24 hours; Refrigerated: 5 days; Frozen: Unacceptable Fresh Tissue: Ambient: Unacceptable; Refrigerated: 2 hours; Frozen: 1 year FFPE tumor tissue: Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable
