| Ordering Recommendation: |
Preferred test for detecting herpes simplex virus (HSV) infection in CSF, neonates, or when rapid diagnostic test for suspected HSV infection is necessary.
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| Methodology: |
Qualitative Polymerase Chain Reaction
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| Performed: |
Sun-Sat
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| Reported: |
1-3 days
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| Specimen Required: |
Collect: Lavender (EDTA), pink (K2EDTA), or serum separator tube. OR Amniotic fluid, bronchoalveolar lavage (BAL), CSF, ocular fluid, tissue, vesicle fluid. OR Endocervical specimen in ThinPrep® Pap Test media.
Specimen Preparation: Separate plasma or serum from cells. Transfer 1 mL serum, plasma, amniotic fluid, BAL, CSF, ocular fluid, or ThinPrep® specimen to a sterile container. (Min: 0.5 mL)
Tissue: Transfer to a sterile container and freeze immediately.
Vesicle Fluid: Transfer to viral transport media (ARUP supply #12884). Available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.
Storage/Transport Temperature: Frozen.
Remarks: Specimen source required.
Unacceptable Conditions: Heparinized specimens.
Stability (collection to initiation of testing): Tissue: Ambient: Unacceptable; Refrigerated: Unacceptable; Frozen: 3 months
All Others: Ambient: 8 hours; Refrigerated: 72 hours; Frozen: 3 months
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