ARUP's Laboratory Test Directory

Varicella-Zoster Virus DFA : 0060290

Mnemonic: VZVFA

Methodology: Direct Fluorescent Antibody Stain
Performed: Sun-Sat
Reported: Within 24 hours
Specimen Required: Patient Prep: Collect vesicle specimen during first 3 days of rash.

Collect: Vesicle swab, tissue, or skin scrapings. Swab should be rolled in base of fresh vesicle to obtain cells.

Specimen Preparation: Immediately transfer specimen to viral transport media (ARUP Supply #12884) and refrigerate (extremely temperature sensitive). Available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.

Storage/Transport Temperature: Refrigerated.

Remarks: Specimen source preferred.

Unacceptable Conditions: Bone marrow, CSF, or whole blood. Calcium alginate, dry, or wood swabs.

Stability (collection to initiation of testing): Ambient: 4 hours; Refrigerated: 72 hours; Frozen: Unacceptable

Reference Interval:
Negative
Interpretive Data: Approximately 20% of samples submitted for VZV are positive for herpes simplex virus.  Testing for herpes simplex virus is NOT included in this test.
Note: Sensitivity of DFA methodology is dependent upon adequacy of the specimen.  If there are fewer than 20 cells, the DFA result will be reported as "sample inadequate."

Culture is recommended for any DFA-negative swabs or inadequate samples.  Refer to Varicella-Zoster Virus DFA with Reflex to Varicella-Zoster Virus Culture (0060282).
CPT Code(s): 87290
Cross References: VZV Antigen Detection (Varicella-Zoster Virus DFA)