ARUP's Laboratory Test Directory

Lymphocyte Transplantation CD3 : 0095949
[ image for: Time Sensitive]
Time Sensitive
  


Mnemonic: CD3

Methodology: Quantitative Flow Cytometry
Performed: Sun-Sat
Reported: 1-2 days
Specimen Required: Patient Prep: Draw specimen before administering immunosuppressive medications.

Collect: Green (sodium or lithium heparin), lavender (EDTA), or pink (K2EDTA).

Specimen Preparation: Transport 5 mL whole blood. (Min: 0.5 mL)

Storage/Transport Temperature: CRITICAL ROOM TEMPERATURE.

Remarks: THIS TEST IS FOR TRANSPLANT PATIENTS ONLY. WBC and lymph percent results with the same draw date and time as the CD3 specimen must be provided by the client. Specimens must be analyzed within 48 hours of collection. Some medications may affect immunophenotyping results and should be listed on the patient test request form.
New York State Clients:
EDTA specimens must be analyzed within 30 hours of collection. Heparin specimens must be analyzed within 48 hours of collection.


Unacceptable Conditions: Clotted, hemolyzed, frozen, or refrigerated specimens. Specimens older than 48 hours.
New York State Clients:
Clotted, hemolyzed, frozen, or refrigerated specimens. EDTA specimens older than 30 hours and heparin specimens older than 48 hours.


Stability (collection to initiation of testing): Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
New York State Clients:
Ambient: 30 hours in EDTA, 48 hours in heparin; Refrigerated: Unacceptable; Frozen: Unacceptable


Reference Interval:
Reports include age appropriate reference intervals and interpretation.
Components Reference Interval
WBC (K/µL)
% Lymph
Absolute Lymph Count
Must be provided by the client.
By report
%CD3
Absolute CD3
By report

Interpretive Data: In this test, the percent CD3 cells are those cells that are positive for both CD3 and the alpha-beta chain of the T-cell receptor and are presumed immunologically competent.

See Compliance Statement A: www.aruplab.com/CS
Note: THIS TEST IS FOR TRANSPLANT PATIENTS ONLY.  Effective immunosuppressive therapy with OKT3 can be documented by verifying CD3 antigen removal using this test.  For immunocompromised patients, order Lymphocyte Subset Panel 4 - T-Cell subsets Percent & Absolute (0095950).
CPT Code(s): 86356 x2
Cross References: T-Cell Transplant Monitoring (Lymphocyte Transplantation CD3)