ARUP's Laboratory Test Directory

BRAF Codon 600 Mutation Detection by Pyrosequencing : 2002498
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Additional Technical Information
  


Mnemonic: BRAF PCR

Ordering Recommendation: Evaluate suspected Lynch syndrome. Use to predict effectiveness of therapies targeting the EGFR pathway. Evaluate thyroid specimens (tissue blocks or formula-fixed, paraffin-embedded cell blocks prepared from fine needle aspirates [FNA]) for BRAF mutations for purpose of diagnosis, prognosis, and possible therapeutics. For FNA direct smears, the preferred test is BRAF V600E Mutation Detection by Allele-Specific PCR with Competitive Probe Blocking, Fine Needle Aspiration (2006516).
Methodology: Polymerase Chain Reaction/Pyrosequencing
Performed: Varies
Reported: 10-14 days
Specimen Required: Collect: Tumor tissue.

Specimen Preparation: Formalin fix (10 percent neutral buffered formalin) and paraffin embed tissue. Protect from excessive heat. Transport tissue block or 5 unstained 5-micron slides. (Min: 3 slides). Tissue block will be returned after testing.
Transport block and/or slide(s) in a tissue transport kit (ARUP supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.

Storage/Transport Temperature: Room temperature. Also acceptable: Refrigerated. Ship in cooled container during summer months.

Remarks: Include surgical pathology report.

Unacceptable Conditions: Less than 25 percent tumor. Specimens fixed/processed in alternative fixatives (alcohol, Prefer®) or heavy metal fixatives. Decalcified specimens.

Stability (collection to initiation of testing): Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable
Interpretive Data: Refer to report.

See Compliance Statement B: www.aruplab.com/CS
CPT Code(s): 88381, 81210, G0452
Cross References: BRAF Gene Mutation Assay,  Melanoma (BRAF Codon 600 Mutation Detection by Pyrosequencing) , BRAF Mutation Analysis, Papillary Thyroid Cancer (BRAF Codon 600 Mutation Detection by Pyrosequencing) , Melanoma, BRAF V600 Mutation, Cobas (BRAF Codon 600 Mutation Detection by Pyrosequencing)