ARUP's Laboratory Test Directory

Hepatitis C Virus RNA Quantitative Real-Time PCR with Reflex to Genotype : 2002685

Mnemonic: HCV QT GR

Ordering Recommendation: Preferred reflex test to confirm active hepatitis C virus (HCV) infection following HCV high-positive screen. Reflex to genotype aids in determining therapy of choice, likelihood of response, and probable therapeutic duration. Order only after positive HCV screen.
Methodology: Quantitative Real-Time Polymerase Chain Reaction/Sequencing
Performed: Sun-Sat
Reported: 6-11 days
Specimen Required: Collect: Lavender (EDTA), pink (K2EDTA), plasma preparation tube (PPT), or serum separator tube.

Specimen Preparation: Separate serum or plasma from cells within 6 hours. Transfer 3.5 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 1.8 mL)

Storage/Transport Temperature: Frozen.

Unacceptable Conditions: Heparinized specimens.

Stability (collection to initiation of testing): On Cells: Ambient: 6 hours; After separation from cells: Refrigerated: 72 hours; Frozen: 6 weeks
Reference Interval:
 
Available Separately Components Reference Interval
0098268Hepatitis C Virus RNA Quantitative, Real-Time PCR Not Detected
0055593Hepatitis C Virus Genotyping By report

Interpretive Data: Refer to individual components.
Note: The limit of quantification for the HCV Quantitative RNA assay is 1.6 log IU/mL (43 IU/mL). If the assay DID NOT DETECT the virus, the test result will be reported as "<1.6 log IU/mL (<43 IU/mL)." If the assay DETECTED the presence of the virus but was not able to accurately quantify the number of copies the test result will be reported as "Not Quantified."

If Hepatitis C Virus RNA Quantitative Real-Time PCR result is greater than or equal to 2.8 log IU/mL, then Hepatitis C Virus Genotyping by PCR & Sequencing will be added. Additional charges apply.
CPT Code(s): 87522; if reflexed, add 87902