ARUP's Laboratory Test Directory

Melanoma Antibody, HMB45 by Immunohistochemistry : 2003935
[ image for: Immunohistochemistry Stain Form Recommended]
Immunohistochemistry Stain Form Recommended
  


Mnemonic: HMB 45 IHC

Methodology: Immunohistochemistry
Performed: Mon-Fri
Reported: 1-3 days
Specimen Required: Collect: Tissue or cells.

Specimen Preparation: Formalin fix (10 percent neutral buffered formalin) and paraffin embed specimen (cells must be prepared into a cellblock). Protect paraffin block and/or slides from excessive heat. Transport tissue block or 5 unstained (3- to 5-micron thick sections), positively charged slides in a tissue transport kit (ARUP supply #47808). Available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787. (Min: 2 slides). If sending precut slides, do not oven bake.

Storage/Transport Temperature: Room temperature or refrigerated. Ship in cooled container during summer months.

Remarks: IMMUNOHISTOCHEMISTRY ORDERING AND SUBMISSION DETAILS: Submit electronic request. If you do not have electronic ordering capability, use an ARUP Immunohistochemistry Stain Form (#32978) with an ARUP client number. For additional technical details, contact ARUP Client Services at (800) 522-2787.

Unacceptable Conditions: Specimens submitted with non-representative tissue type. Depleted specimens.

Stability (collection to initiation of testing): Ambient: Indefinitely, Refrigerated: Indefinitely, Frozen: Unacceptable
Note: All stains will be handled as "Stain and Return" unless a consultation is requested. To request a consultation, submit the pathology report, all associated case materials (clinical history, blocks, slides, etc.), and the Anatomic Pathology requisition form (form # 32960) in place of the Immunohistochemistry Stain Form.
CPT Code(s): 88342
Cross References: HMB 45 (Melanoma Antibody, HMB45 by Immunohistochemistry)