#ExistPATIENTPREP>Patient Prep: Indications: Detection of malignant cells in nipple discharge specimens.
#ExistCollect>Collect: Direct smear of nipple discharge.
Supplies: Two clean glass slides (single-end frosted), fixative (spray fixative or 95% ethanol), test request form.
Collection Procedure: Label the two slides with the patient's first and last name, date of birth and specimen site in pencil on the frosted end. Collect a small amount of nipple secretion directly onto one of the slides. Oppose a second glass slide onto the first, allowing the collected material to provide surface tension between the two slides, and then gently and quickly pull the two slides apart in a horizontal motion to distribute the material in a thin film over both slides. The smears should be immediately fixed either by spray fixative or in 95% ethyl alcohol to prevent air-drying. Submit the specimen and the completed test request form to the Cytopathology Laboratory.
#ExistConditions>Unacceptable Conditions: Refer to the Specimen Rejection section in the Specimen Transport Guide.
#ExistStability>Stability (collection to initiation of testing): Fixed slides: Indefinitely