| Methodology: |
Microscopy
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| Performed: |
Mon-Fri
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| Reported: |
1-5 days
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| Specimen Required: |
Patient Prep: Indications: Cytologic screening for anal squamous intraepithelial lesions (ASIL).
Collect: Anal sampling.
Supplies: Glass microscope slide for smears or, preferably, collected in SurePath™ or PreservCyt® collection fluid. Available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.
Also required: ARUP Cytology test request form and a black lead lab pencil.
Collection Procedure: To obtain an anal sampling, moisten the Dacron® swab with water, not lubricant. The Dacron® swab should be inserted approximately 1.5 to 2 inches into the anal canal. It is important to use Dacron® and not a cotton swab, as cells tend to cling to cotton and do not release easily into cytology collection fluids. Once inserted deep enough into the anus (necessary in order to collect both rectal columnar and anal squamous cells) the swab should be pulled out, applying some pressure to the wall of the anus, rotating the swab in a spiral motion along the way. The cells collected can be placed in a cytology collection fluid (preferred) or spread on a glass slide and immediately spray-fixed. The preferred method of collection is in SurePath™ collection fluid. The collection device should be thoroughly rinsed and swirled in the vial. The vial should be labeled with the patient's first and last name and collection date, placed in a specimen bag, and shipped to the Cytology Lab. The specimen must be accompanied by an ARUP Cytology test request form with the requested test marked.
Storage/Transport Temperature: If collected in SurePath™, Cytolyt®, or CytoRich™:
Unacceptable Conditions: Specimens submitted in expired reagents. Refer to the Specimen Rejection section of the Specimen Transport Guide.
Stability (collection to initiation of testing): SurePath™: Ambient: 4 weeks
PreservCyt®: Ambient: 3 weeks
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